Fig. 1.

Up-regulation of K18 by Ese-2. A549 and IB3-1 cells were co-transfected with a SEAP reporter construct and expression constructs Ese-2, Ese-2-ets, Ese-2-pnt, both Ese-2-ets and Ese-2-pnt, or the mammalian expression vector pcDNA3 (negative control). Transcription of SEAP was driven by the K18 minimal promoter with the K18 first intron inserted upstream of the SEAP gene. The Ese-2 construct was designed to express full length Ese-2. The Ese-2-ets construct was designed to express a protein containing the last 123 amino acids of Ese-2, which encodes the ETS domain. The Ese-2-pnt construct was designed to express a protein containing the first 145 amino acids of Ese-2, which encodes the PNT domain. Media was processed for SEAP assays and the results of 3 separate experiments, each done in triplicate, are shown as Mean ± SE. The negative control was arbitrarily assigned a value of 1, and all other data was normalized to this value. Bars represent SE. ANOVA was performed to establish statistical significance between means. Data with a significantly different mean is marked with a star (*), (P<0.05, Dunnet two-sided test).