Tab. 1.
Oligonucleotides used in EMSAs.
| Oligonucleotide | Sequence of top strand (5′-3′) | Sequence of bottom strand (5′-3′) |
|---|---|---|
| A | ccAATGACGGAAAGAGGCAc | gTGCCTCTTTCCGTCATTgg |
| B | GTGGTTATGGAATGACGGTC | GACCGTCATTCCATAACCAC |
| C | GCGCGGATGGAAATCCAGG | CCTGGATTTCCATCCGCGC |
| D | CTCTCCTGGGAAAGAGCCCTC | GAGGGCTCTTTCCCAGGAGAG |
| E | GAGAGGGGGAAGGGGACAG | CTGTCCCCTTCCCCCTCTC |
| F | CTTTACAGAGGAAGTGGACAG | CTGTCCACTTCCTCTGTAAAG |
| G | GGAGGTAAGGAAAGGCCTG | CAGGCCTTTCCTTACCTCC |
| H | GCGTCTGGCGGAATGGGGAC | GTCCCCATTCCGCCAGACGC |
| I | CTCCCAAAGGAAGGCCTCTTG | CAAGAGGCCTTCCTTTGGGAG |
| J | GTGCACTAGGGAAAAAATGCC | GGCATTTTTTCCCTAGTGCAC |
| K | GAGGGCCAGGAAGAGGACTTG | CAAGTCCTCTTCCTGGCCCTC |
| L | CCTCTTGGGAAGCCTCAGC | GCTGAGGCTTCCCAAGAGG |
| M | GGATCTGGGAATCCAGGTCC | GGACCTGGATTCCCAGATCC |
| N | CGATCCAGGAAAGGAGGATCG | CGATCCTCCTTTCCTGGATCG |
| O | CGAAGGAGGGAATGGGGTGG | CCACCCCATTCCCTCCTTCG |
| P | GGTCCCAGGAATAGCCAGAGG | CCTCTGGCTATTCCTGGGACC |
| Q | GGACCAGGAAGTTTTCACTAGG | CCTAGTGAAAACTTCCTGGTCC |
| R | GCTATTCATGGAACAACCTCTC | GAGAGGTTGTTCCATGAATAGC |
| E74 | TCGAGTAACCGGAAGTAACTCAG | CTGAGTTACTTCCGGTTACTCGA |
Note: Core recognition sequence in oligonucleotides A-R, listed in the order they appear in the first intron of K18 and oligonucleotide E74 are underlined; small font indicates bases that were introduced to avoid more than one core sequence in an oligonucleotide.