Figure 4.

NF-κB binding to the ESE-1 and/or ESE-3 promoters in vivo. (A) Schematic diagrams of the PCR primer positions as indicated by arrows. Ovals represent the NF-κB binding sites. (B, C) Chromatin immunoprecipitation (ChIP) assays. Chromatin preparations that were made from BEAS-2B or A549 cells stimulated with IL-1β and TNF-α (at 5 ng/ml each) for the time periods indicated were precipitated using antibodies against NF-κB p65 or p50. ‘Pre’ represents pre-immune rabbit serum and ‘input’ represents a PCR control using chromatin without precipitation.