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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Cell Res. 2008 Jun;18(6):649–663. doi: 10.1038/cr.2008.57

Figure 5.

Figure 5

Overexpression of ESE-1 or ESE-3 affects promoter activity differently. (A) Overexpression of ESE-1 or ESE-3 down-regulates ESE-1 induction by cytokines. A549 cells were co-transfected with 1 μg of ESE-1 promoter reporter plasmid and 0.5 μg of pCDNA3 (vec), or pCDNA3 containing ESE-1 or ESE-3. At 24 h after transfection, the cells were cultured in new media with or without the presence of IL-1β and TNF-α (at 10 ng/ml each) for 24 h. (B) Overexpression of ESE-1 upregulates ESE-3 expression. A549 cells were co-transfected with 1 μg of ESE-3 promoter SEAP reporter plasmid and 0.5 μg of an expression vector, pCDNA3 (vec), or pCDNA3 containing ESE-1 or ESE-3. (C) Dose-dependent enhancement of ESE-3 expression by ESE-1. A549 cells were co-transfected with 1 μg of ESE-3 promoter reporter plasmid and different amount of ESE-1 expression plasmid as indicated. SEAP activity was measured as relative light units using a luminometer. Values shown are the mean ± SEM (n = 3) from one representative of three independent experiments.

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