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. 2015 Jan 31;6(14):11779–11793. doi: 10.18632/oncotarget.3151

Figure 7. COP1 accelerates tumor formation.

Figure 7

(A) 14-3-3σ antagonizes COP1-mediated cell motility. U2OS/RFP-COP1 and U2OS/RFP-vector cells were plated for transwell assay. Stably expressing U2OS/RFP-COP1 cells were left uninfected, infected with Ad-β-gal or Ad-HA-14-3-3σ for 1 day. Infected cells were plated for transwell assay. Number of migrated cells was quantitated and presented as bar graphs. Error bars represent 95% confidence intervals. Three asterisk, p < 0.001 (Student's t-test, n = 3). Scale bar, 50 μm. (B) 14-3-3σ hinders COP1-mediated tumorigenicity. Indicated cells were injected subcutaneously into nude mice. Tumor volumes were measured at the indicated days. Error bars represent 95% confidence intervals. One asterisk, p < 0.05. Three asterisk, p < 0.001 (Student's t-test, n = 6). (C) Serial tumor sections from the experiment were stained with anti-cleaved Caspase 3 and anti-Ki67. (D) Model of CSN6-COP1-p27 axis in regulating chromosome stability.