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. 2015 Mar 18;6(14):11806–11819. doi: 10.18632/oncotarget.3688

Figure 5. Effect of STS in combination with chemotherapy on reactive oxygen species production and apoptosis of colon carcinoma cells.

Figure 5

Panel A shows the oxidation state of the dye Dichlorodihydrofluoresceindiacetate (DCFDA) as a method to assess the level of reactive oxygen species (ROS) in CT26 colon carcinoma cells, undergoing STS (48 hours) or under standard conditions, and treated with or without 100μM oxaliplatin (OXP) for 24 hours. Results are expressed as mean of the percentage of DCFDA positive cells from three different experiments. Panel B shows a representative dot plot of CT26 cells, undergoing STS or under standard conditions, treated with or without OXP (100 μM) and stained with FITC Annexin V and propidium iodide (PI). Red squares indicate the percentage of Annexin+/PI− and Annexin+/PI+ apoptotic cells (early and late apoptosis). Panel C shows the percentage of Annexin+/PI− and Annexin+/PI+ apoptotic CT26 cells expressed as mean value ± SD from three different experiments performed. P value was calculated using Unpaired t test with Welch's correction. *: P<0.05; **: P<0.01. Panel D shows a representative heat map of proteins annotated by Gene Ontology as involved in apoptosis. Data are expressed as ratios over mean values for the four conditions (STS, OXP, STS+OXP; red = expression above mean, yellow = expression at mean; blue = expression below mean).