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. 2015 Apr 25;6(14):11863–11881. doi: 10.18632/oncotarget.3787

Figure 4. Absence of jnk2 increases the luminal cell population and BRCA1 expression in p53ko tumors.

Figure 4

A. p53ko tumors were measured until reaching 1.5 cm diameter (n = 22 p53ko;jnk2wt, n = 18 p53ko;jnk2ko, Log rank test); B-C. p53ko tumors were immunostained and Ki-67+ and CK8/18+ cells were quantified (n = 5); D-E. Expression of basal (red) and luminal (blue) markers was measured in p53ko tumors (n = 8, E) and p53ko cell lines F. Western blot of BRCA1 expression in p53ko cells; G. p53ko cells were transfected with Brca1 promoter (BRCA1PR) or promoterless control (PRless) luciferase plasmids and assayed for promoter activity; H. Correlation of Brca1 expression and EMT-related gene expression was assessed in human tumors (UNC308, n = 308, and COMBINED855, n = 855) and p53ko mouse tumors (n = 15, P.C. = Pearson Correlation). A nonparametric, two-tailed t-test was used to detect statistical differences between two groups. The Pearson's correlation was performed using data in H. *p < 0.05, **p < 0.001, ***p < 0.0001.