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. 2015 May 18;6(14):11979–11993. doi: 10.18632/oncotarget.3979

Figure 6. Measurements of oxidative damage and mitochondrial health after treatment with AT1R blocker.

Figure 6

Western blot analyses of cardiac protein extracts from aged WT (aWT) A., young IL-10−/− (yKO) F., and aged IL-10−/− (aKO) K. mice with (+L) or without losartan treatment using antibodies against Nitrotyrosine (NT) and Nix. Actin was used as a loading control. Relative expression was calculated for the Western blots displayed in arbitrary units (AU) for aWT B–C., yKO G–H. and aKO L–M. Relative mitochondria DNA (mtDNA) copy number as a measure of the ratio between cytochrome B and GapDH for aWT D., yKO I. and aKO N. ATP concentration of cardiac muscle of aWT E., yKO J. and aKO O. Data are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.