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. 2015 Jan 22;6(14):11994–12008. doi: 10.18632/oncotarget.2740

Figure 2. Phosphorylation of AKT and ERK1/2 is increased in human PCa 22Rv1 cells overexpressing CRIPTO.

Figure 2

(A) Western blot shows that 24h transfection with CR-1 increases phosphorylation of AKT and ERK1/2 in 22Rv1 cells but not in LNCaP cells. (B) The activation of AKT, ERK1/2, SRC, SMAD2 was compared in 22Rv1/vector and 22Rv1/CR-1 stable transfectants. (C) siRNA targeting of CR-1 in 22Rv1/CR-1 cells downregulates phospho-AKT and phospho-ERK1/2 at 72hr. The number below each lane is the quantified fold change (as evaluated by densitometry analysis) when compared with the first lane and when applicable after normalization to beta-actin (total ERK, AKT and CR-1), total ERK (p-ERK/1/2), or total AKT (p-AKT).