Appendix 1. IHC Thresholds.

Protein	Threshold for Overexpression (Intensity and %expression in tumor)
AR	≥ 1+ and ≥ 10%
cKIT	≥ 2+ and ≥ 30%
cMET	≥ 2+ and ≥ 50%
EGFR	2+ and = 10% or 1+ and < 10%; current = H-score, < 200 or ≥ 200
ER	≥ 1+ and ≥ 10%
PR	≥ 1+ and ≥ 10%
HER2	≥ 3+ and > 10%
PD-1	≥ 1+ (TIL Count/High power field with 40x objective)
PD-L1	≥ 2+ and ≥ 5%
PDGFRA	≥ 2+ and 30%
PGP	≥ 1+ and ≥ 10%
SPARC	≥ 2+ and ≥ 30%
TLE3	≥ 2+ and ≥ 30%
TOPO1	≥ 2+ and ≥ 30%
TOP2A	≥ 1+ and ≥ 10%
TUBB3	≥ 2+ and ≥ 30%
Protein	Threshold for Loss (Intensity and %expression in tumor)
ERCC1	< 2+ or ≤ 3+ and < 10% or = 2+ and < 50%
MGMT	= 0+ or ≤ 35%
PTEN	= 0+ or ≤ 50%
RRM1	< 2+ or < 50%