Skip to main content
. 2015 Apr 14;6(14):12436–12451. doi: 10.18632/oncotarget.3814

Figure 2. Cytotoxicity, uptake and cellular localization of MH3-B1/rGel after 4 hrs incubation.

Figure 2

Relative cell viability (MTT) of SK-BR-3 (A) and SKOV-3 (B) following 4 hrs incubation with MH3-B1/rGel, rGel and MH3-B1, representative sigmoidal curves of three experiments, (fit model: a/(1+exp(−(x-x0)/b))), error bars = SD. (C): Cellular uptake of 2 nM Alexa488-MH3-B1/rGel following 4 hrs incubation in SK-BR-3 and SKOV-3 cells, representative flow cytometry charts of three experiments. (D): Total cellular Alexa488-MH3-B1/rGel fluorescence, average of three experiments, error bars = SE. (E): SK-BR-3 and SKOV-3 cells following 4 hrs incubation of 2 nM Alexa-488-MH3-B1/rGel. Alexa488-MH3-B1/rGel (green), LTR (blue) and colocalization of the two (white). The confocal micrographs are representative of three independent experiments. The colocalization of Alexa488-MH3-B1/rGel with LTR was calculated by the Manders' thresholded coefficient from the total cellular accumulation measured (F) and the Pearson's correlation coefficient was used to calculate the correlation between Alexa488-MH3-B1/rGel and LTR based on fluorescence intensities/pixel (G), F and G shows average of three experiments, error bars = SE.