Figure 5. Tumor growth and metastasis in vaccinated mice challenged with B16F10/shTGF-β1 cells plus minus miR200c agomir.

A. Images shows the tumor sizes dissected from the differently treated mice on Day 51. After the mice were initially immunized s.c. with 1×10⁶ B16F10/GPI-IL-21 inactivated tumor vaccine the mice were challenged by the differently treated B16F10 cells as described in the section of materials and methods. B. The quantification analysis of tumor sizes (*p < 0.05, ** p < 0.01 and *** p < 0.001). C. Tumor free mice during 51 days. D. Images of tumor metastatic focus in mouse lungs. E. The quantification analysis of lung metastatic focus counts (HPF, ×400). Data are represented as mean +/− SEM; refer to the statistical differences as indicated.