Figure 1. Comparison of the levels of D-2-HG and L-2-HG between IDH1/2 wildtype and mutant IDH1/2 chondrosarcoma cell lines.
A) IDH1 mutation in cell line JJ012. Mutation status for all cell lines was confirmed and the expression of the mutant allele was shown by Sanger sequencing of the cDNA. B) intracellular levels of D-2-HG and L-2-HG normalized to total protein concentration. Elevated levels of D-2-HG up to a 1000 fold were observed in mutant IDH1/2 cell lines compared to the IDH1/2 wildtype chondrosarcoma cell lines. C) Elevated levels of D-2-HG were also found in the conditioned media, D-2-HG levels were normalized to L-2-HG concentration. Error bars represent mean ± SD of measurements in three different passages.