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. 2015 Mar 30;6(14):12587–12602. doi: 10.18632/oncotarget.3494

Figure 2. PAICS and IMPDH2 are overexpressed in prostate cancer patients.

Figure 2

A. Schematic overview of the de novo purine biosynthesis pathway with direct MYC targets highlighted in yellow. B. Real-time PCR results on clinical samples. Total RNA was collected from prostate biopsies of patients suffering from benign hyperplasia (BPH), prostate cancer (PCa) or castrate-resistant prostate cancer (CRPC). The expression levels of MYC, PAICS and IMPDH2 were measured using real-time PCR and normalized to the expression of TATA-box binding protein (TBP). The line displays the mean value and significance was determined using one-way analysis of variation (ANOVA) with Bonferroni's multiple comparison test. n = 15–27 C. Immunohistochemistry data. Staining intensities of PAICS and IMPDH2 in patient biopsies with BPH, PCa CRPC, hormone-naïve, short and long NHT were assessed. Intensities divided into four groups (negative = 0, weak = 1, moderate = 2 and strong = 3) and used for subsequent analysis. Statistical analysis was performed using one-way analysis of variation (ANOVA) with Bonferroni's multiple comparison test. n = 20–113 cores D. Representative images of the TMAs for PAICS (left) and IMPDH2 (right).