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. 2015 Mar 30;6(14):12587–12602. doi: 10.18632/oncotarget.3494

Figure 3. Inhibition of IMPDH2 impairs the proliferation of prostate cancer cells and shows additive effects with established anti-androgens.

Figure 3

A. Cell viability results of siRNA treated cells. Cells were transfected with 25 nM (control 25 nM, IMPDH1 and IMPDH2 #1) or 50 nM (control 50 nM, PAICS, IMPDH2 #2 and MYC) for 72 h and cell viability relative to siCTRL 50 nM was determined using a MTS-based assay. n = 2–4 B. Cell viability results of MPA treated cells. Cells were allowed to attach for 48 h prior to treatment with indicated doses of MPA for 72 h. Cell viability relative to vehicle control was determined using a MTS-based assay. n = 2–4 C. Cell viability results of siRNA and Abiraterone/MDV3100 treated cells. Cells were transfected with 25 nM IMPDH2 #1, 50 nM IMPDH2 #2 siRNA or equal amounts of siCTRL for 48 h. Following treatment with the indicated drugs for another 72 h, viability relative to DMSO and siCTRL was assessed using a MTS-based assay. Doses for LNCaP were 1 μM Abiraterone and 1 μM MDV3100, and for VCaP 1 μM Abiraterone and 100 nM MDV3100 n = 1–4 D. Cell viability results of MPA and Abiraterone/MDV3100 treated cells. Cells were allowed to attach for 48 h prior to treatment with indicated drug combinations for 72 h. Cell viability relative to vehicle control was determined using a MTS-based assay. Doses for LNCaP were 10 μM MPA, 1 μM Abiraterone and 1 μM MDV3100, and for VCaP 5 μM MPA, 1 μM Abiraterone and 100 nM MDV3100 n = 3–4 E. Cell viability results of MPA treated parental and MDV3100-resistant LNCaP. Cells were allowed to attach for 48 h prior to treatment with the indicated doses of MPA for 72 h. Cell viability relative to vehicle control was determined using a MTS-based assay. n = 4 F. Cell viability results of siRNA and MPA treated cells. Cells were transfected with 50 nM MYC or control siRNA for 48 h. Following treatment with the indicated doses of MPA (LNCaP 5 and 10 μM, VCaP 5 and 10 μM and LNCaP abl 1 and 10 μM) for another 72 h, viability was assessed using a MTS-based assay and normalized to the respective DMSO control. n = 3.