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. 2015 Mar 29;6(14):12603–12624. doi: 10.18632/oncotarget.3687

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Copyright: © 2015 Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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In vitro HEK293 cell & EBV-GFP infection assay followed FACS analysis was conducted to determine if treatments of quercetin or isoliquiritigenin affect maintenance of EBV latency in SNU719 cells. Measurement of the FITC emission from FACS Aria III helps determine the cytometric profile of EBV latency in HEK293-EBV-GFP cells. HEK293-EBV-GFP cells are HEK293 cells that were latently infected EBV recombinant viruses and selected with hygromycin B. Compared to DMSO treatment (negative control), quercetin treatment (62 μM) decreased FITC emission up to 11%, but isoliquiritigenin treatment (45 μM) increased FITC emission up to 18%. HEK293 cells were subjected to FACS analysis as an internal negative control. ISL and QST stands for isoliquiritigenin and quercetin, respectively.