Figure 3. siRNA silencing of USP22.

HepG2 cells were transfected with USP22 siRNA and negative control siRNA. At 48 h post-transfection, changes in the mRNA and protein levels of USP22 were determined using RT-PCR (A) and Western blot (B) analyses. β-actin was used as internal control for Western blot and RT-PCR. (C) Relative protein levels were quantified using Image J (NIH, Bethesda, MO, USA) and normalized versus internal control. Three independent experiments were performed and representative results are shown.