Figure 5. USP22 gene silencing leads to HCC cell apoptosis.

HepG2 cells were transfected with USP22 siRNA and negative control siRNA. The cells were analyzed at 24 h post-transfection. (A) Flow cytometry were performed to analyze cell apoptosis rate. Each independent experiment was performed 3 times. (B) USP22 expression was observed in HCC cells via immunohistochemical staining with an anti-USP22 antibody under a confocal laser scanning microscope.