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. 2015 Mar 30;6(14):12654–12667. doi: 10.18632/oncotarget.3705

Figure 6. USP22 gene silencing induces mitochondrial apoptosis in HepG2 cells.

Figure 6

At 48 h post-transfection with USP22 siRNA and control siRNA in HepG2, the mitochondria and protein in the cytoplasm were isolated and subjected to Western blot analysis. β-actin was used as internal control. (A). Protein levels of caspase-3(Cas-3), cleaved caspase-3 (C-Cas-3), Bcl-2, Bax, Bcl-XL, and cytochrome c (Cyt C) in the cytoplasm; (B) Relative protein expression levels in the cytoplasm were quantified using Image J and normalized versus international control; (C). Protein levels of Bcl-2, Bax and CytC in the mitochondria.(D) Relative protein expression levels in the mitochondria were quantified using Image J and normalized versus international control density. Three independent experiments were performed and representative results are shown.