Figure 5. Metformin treatment led to aberrant expression of invasion/migration-related genes.

(A) Heatmap representing hierarchical clustering of all genes that displayed a 1.5-fold or greater difference in transcript levels in metformin-treated BGC823 cells with a concentration of 20 mM compared to controls at days 0, 24 h, and 48 h (B) Significant gene ontology (GO) terms retrieved by clusters D–G. (C) Pearson correlation network of the metformin action course generated using the Spring-embedded algorithm in Cytoscape. Partial genes in enriched GO categories are represented in the network. Nodes represent genes, and connections represent correlation coefficient.