Figure 2. Prediction of spatially resolved proteome allocation and limitations.
A) The expanded scope of reconstruction content advances the predictions possible with constraint-based models from network flux states (with M-Models) to the spatially resolved molecular composition of a cell (with ME-Models).
B) ME-Models predict the gross macromolecular composition of the cell and the detailed allocation of the proteome. Additionally, the effects of proteome limitations can be accounted for, including the prediction of optimal substrate uptake rates and specific pathway shifts from carbon-limited to carbon-excess environments.