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. 2015 Jul 7;10(7):e0131102. doi: 10.1371/journal.pone.0131102

Fig 5. Embryonic lineage preference in hESCs after downregulation of epigenetically-defined biomarkers.

Fig 5

Expression at 72 hours after RH1 hESCs were treated with siRNAs indicated. RT-qPCR data showing log10 fold change in expression of the indicated embryonic lineage marker gene. Changes are relative to GAPDH expression, normalised to IDS-NULL-treated RH1 cells. Asterisks indicate levels of statistical significance (unpaired t-test; *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001). ND: Not Detected, even at 40 cycles of PCR.