Skip to main content
. 2015 Jul 7;10(7):e0132339. doi: 10.1371/journal.pone.0132339

Fig 6. Trimeric complex formation between HPV16 E6, p53 and pep11** or E6AP, in GST pull-down analyses.

Fig 6

(A) p53 was expressed in H1299/K3 cells and pull-down assays were performed with GST-tagged HPV16 E6 (GST-16E6) in the presence of no peptide, synthetic pep11** or synthetic pep11**m. E6AP and p53 bound to GST-16 E6 are indicated. (B) The H1299/K3 lysate was pre-cleared from endogenous E6AP protein by pre-incubation with GST-16 E6. E6AP and p53 bound to GST-16 E6 are indicated.