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Effect of Ang II on mineralized nodule formation. ROS17/2.8 cells were cultured with 0 (control) or 10–6 M Ang II for up to 7 days. Mineralized nodule formation (A) was examined by Alizarin Red staining on days 3, 5, and 7 of culture, and the calcium content in mineralized nodules (B) was determined using a calcium assay kit
Each bar indicates the mean ± SD from three independent experiments. **p < 0.01 (Ang II treatment vs. control).
