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Effect of losartan on Runx2, Msx2, AJ18, and osteocalcin mRNA expression. ROS17/2.8 cells were cultured with 0 (control) or 10–6 M Ang II in the presence or absence of the AT1 receptor blocker (losartan) for 5 days. mRNA expression levels of Runx2 (A), Msx2 (B), AJ18 (C), and OCN (D) were determined using real-time PCR
Each bar indicates the mean ± SD from three independent experiments. *p < 0.05.
