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The RT-PCR analysis of stem and germ cell specific markers in colony forming cells. Bands related to the expression of Oct-4, Fragilis, C-kit, Nanog, Mvh and Dazl were observed on gel in the right predicted size. Cells did not express Scp3 and Gdf9 (data not shown). β-Actin as a housekeeping gene was also studied. Expression profile of ovarian cells from adult ovaries and liver cells were utilized as positive and negative controls, respectively
