FIG 4.

Effects of pH on the activities and stabilities of TVN1315 (A and C) and Ta0286 (B and D). (A and B) The activity was measured at 50°C in appropriate buffers at different pHs, including 50 mM Gly-HCl buffer (pH 2.5 to 4.0, circle) and 50 mM acetate buffer (pH 3.3 to 4.5, square). (C and D) Trehalases were incubated at 4°C for 60 min at various pHs, including 50 mM Gly-HCl buffer (pH 3 to 4, circle), 50 mM acetate buffer (pH 4 to 6, square), 50 mM morpholineethanesulfonic acid–NaOH buffer (pH 5 to 7, triangle), 50 mM Tris-HCl buffer (pH 7 to 9, rhombus), and 50 mM carbonate-NaOH buffer (pH 10 to 11, cross). The remaining activity was measured at 50°C and pH 3.7. The average values for TVN1315 (A and C; open symbols) and Ta0286 (B and D; closed symbols) with error bars are represented as the enzymatic activity. Experiments were performed in triplicate.