. 2015 Jul 7;81(15):4920–4931. doi: 10.1128/AEM.00956-15

TABLE 1.

Steady-state kinetic parameters of trehalases using trehalose as the substrate at 50°C

Enzyme	k_cat^a (s⁻¹)	K_m^a (mM)	k_cat/K_m (s⁻¹ mM⁻¹)	Buffer^b
TVN1315	63.0 ± 0.4	48.7 ± 1.4	1.29	Gly-HCl, pH 3.7
	60.4 ± 0.8	49.0 ± 2.0	1.23	Acetate, pH 3.8
Ta0286	66.7 ± 1.3	40.2 ± 1.5	1.66	Gly-HCl, pH 3.7
	59.1 ± 1.7	44.1 ± 1.2	1.34	Acetate, pH 3.8

Values for k_cat and K_m (means ± standard deviations) are based on results using trehalose (0 to 270 mM) as the substrate. Experiments were performed in duplicate or triplicate.

Buffer solution (50 mM).