FIG 5.

Confocal microscopic images of biofilms. Biofilms of S. mutans UA159 and mutant strains were grown in BM supplemented with 20 mM glucose for 24 h (A) or 48 h (B) at 37°C in a 5% CO₂ atmosphere. The data presented are representative of those from three independent experiments. The distance between two asterisks is 5 μm per side. Small images at the bottom present biofilm thickness. See the Materials and Methods section for details on labeling and image analysis.