TABLE 1.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Genotype or relevant characteristic(s) | Source or reference(s) |
|---|---|---|
| Streptococcus mutans strains | ||
| UA159 | Wild type | Laboratory stock |
| ΔackA | ΔackA::NPKmr | This study |
| Δpta | Δpta::NPKmr | 21 |
| ΔptaE | Δpta::NPErmr | 21 |
| ΔptaE ΔackA | Δpta ΔackA::NPKmr Ermr | This study |
| ΔrelA | ΔrelA::NPErmr | 31 |
| ΔrelP | ΔrelP::NPKmr | 31 |
| ΔrelQ | ΔrelQ::NPKmr | 31 |
| ΔrelRS | ΔrelRS::NPErmr | 31 |
| KB12 | Δpta::NPKmr carrying pKB101 | 21 |
| KB034 | ΔackA::NPKmr carrying pKB019 | This study |
| Plasmids | ||
| pDL278 | E. coli-Streptococcus shuttle vector, Spr | 38, 55 |
| pKB101 | pta coding region plus promoter region cloned into pDL278 | 21 |
| pKB019 | ackA coding region plus promoter region cloned into pDL278 | This study |
a
The integration vector pJL84 is described elsewhere (43).