FIG 6.

EMSAs of AveT binding to the aveM-sav_7491 intergenic region and transcriptional analysis of aveM and sav_7491 by real-time RT-PCR. (A) Schematic representation of probe aveM_sav_7491_int (a 178-bp DNA fragment covering the aveM-sav_7491 intergenic region), used for EMSAs. (B) EMSAs of the interaction of probe aveM_sav_7491_int with purified His₆-AveT protein. Each lane contained 0.15 nM labeled probe. The concentrations of His₆-AveT for probe aveM_sav_7491_int were 0.005, 0.01, 0.05, and 0.1 μM. (C) aveM and sav_7491 transcription levels in the WT, ΔaveT, and OaveT strains. P values were determined by Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.