TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| S. avermitilis | ||
| ATCC 31267 | WT strain | Laboratory stock |
| A-178 | An industrial strain | Qilu Pharmaceutical |
| ΔaveT | aveT deletion mutant | This study |
| CaveT | aveT-complemented strain | This study |
| OaveT | aveT-overexpressing strain | This study |
| OaveT/A-178 | aveT-overexpressing strain based on A-178 | This study |
| WT/pKC1139 | WT strain carrying empty vector pKC1139 | This study |
| WT/pSET152 | WT strain carrying empty vector pSET152 | This study |
| ΔaveM | aveM deletion mutant | This study |
| CaveM | aveM-complemented strain | This study |
| OaveM | aveM-overexpressing strain | This study |
| ΔaveM/A-178 | aveM deletion mutant based on A-178 | This study |
| ΔaveTaveM | aveT aveM double deletion mutant | This study |
| OpepD2 | pepD2-overexpressing strain | This study |
| Δaco | aco deletion mutant | This study |
| E. coli | ||
| JM109 | General cloning host for plasmid manipulation | Laboratory stock |
| ET12567 | Methylation-deficient strain | 33 |
| BL21(DE3) | Host for protein overexpression | Novagen |
| Plasmids | ||
| pKC1139 | Multiple-copy, temperature-sensitive E. coli-Streptomyces shuttle vector | 48 |
| pSET152 | Integrative E. coli-Streptomyces shuttle vector | 48 |
| pET-28a (+) | Vector for protein overexpression in E. coli | Novagen |
| pJL117 | pIJ2925 derivative carrying the Streptomyces strong constitutive promoter ermE*p | 49 |
| pΔaveT | aveT deletion vector based on pKC1139 | This study |
| pKC1139-ermp-aveT | aveT-overexpressing vector based on pKC1139 | This study |
| pSET152-aveT | aveT-complemented vector based on pSET152 | This study |
| pET28-aveT | aveT-overexpressing vector based on pET-28a (+) | This study |
| pΔaveM | aveM deletion vector based on pKC1139 | This study |
| pKC1139-ermp-aveM | aveM-overexpressing vector based on pKC1139 | This study |
| pSET152-aveM | aveM-complemented vector based on pSET152 | This study |
| pKC1139-ermp-pepD2 | pepD2-overexpressing vector based on pKC1139 | This study |
| pΔaco | aco deletion vector based on pKC1139 | This study |