TABLE 1.
Hydrolysis of DON-3G by fungal and bacterial β-glucosidasesd
| Preparation/source | Catalog no. | GH family | Protein in assay (mg ml−1) | DON-3G hydrolysis (%) | Hydrolysis measured as: |
|
|---|---|---|---|---|---|---|
| μmol h−1 mg−1 | μmol h−1 ml−1 | |||||
| Aspergillus nigera (AnBgl) | E-BGLUC | 3 | 0.19 | 24 | 6.9 × 10−3 | 5.3 × 10−3 |
| Agrobacterium sp.a | E-BGOSAG | 1 | 0.48 | 1.1 | 1.3 × 10−4 | 2.4 × 10−4 |
| Thermotoga maritimaa | E-BGOSTM | 1 | 0.78 | 21 | 1.5 × 10−3 | 4.6 × 10−3 |
| Phanerochaete chrysosporiuma (PcBgl) | E-BGOSPC | 3 | 1.1 | 88 | 4.3 × 10−3 | 1.9 × 10−2 |
| Glucosidase from almondsb,c | G0395 | 5 | Not detectable | |||
| Glucosidase from A. nigerb,c | 49291 | 5 | 3.3 | 3.6 × 10−5 | 7.3 × 10−4 | |
| Novozyme 188b | C6105 | Not specified | 38 | 8.4 × 10−3 | ||
| Lactobacillus brevis (LbBgl) | 3 | 1 | 100 | >5.12 × 10−3 | >2.2 × 10−2 | |
a
From Megazyme International (Wicklow, Ireland); concentrations in assays result from the supplied solution.
b
From Sigma-Aldrich (Vienna, Austria).
c
Supplied in solid form.
d
Hydrolysis of DON-3G was performed with a 4-h reaction time, pH 7 (100 mM Tris-Cl), at 37°C.