TABLE 4.
Buffer | Relative enzyme activity (%) |
|
---|---|---|
LbBgl | BaBgl | |
Tris (100 mM) | 100 ± 0 | 100 ± 2 |
McIlvainea | 158 ± 2 | 174 ± 1 |
Phosphate buffer (100 mM) | 127 ± 2 | 143 ± 1 |
MOPSb (100 mM) | 163 ± 2 | 158 ± 2 |
HEPES (100 mM) | 145 ± 0 | 132 ± 1 |
Succinic acid (100 mM) | 124 ± 2 | 135 ± 2 |
Britton-Robinson bufferc | 136 ± 1 | 141 ± 1 |
Citrate-phosphate buffer according to reference 35.
Morpholinepropanesulfonic acid.
Described in reference 28.
Relative activities of LbBgl and BaBgl in organic and inorganic buffers at pH 7 were determined with pNP-β-d-glucopyranoside at 37°C. All values represent the means from triplicate determinations ± standard deviations.