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. 2015 Jul 7;81(15):4885–4893. doi: 10.1128/AEM.01061-15

TABLE 4.

Relative activities of LbBgl and BaBgl in organic and inorganic buffers at pH 7d

Buffer Relative enzyme activity (%)
LbBgl BaBgl
Tris (100 mM) 100 ± 0 100 ± 2
McIlvainea 158 ± 2 174 ± 1
Phosphate buffer (100 mM) 127 ± 2 143 ± 1
MOPSb (100 mM) 163 ± 2 158 ± 2
HEPES (100 mM) 145 ± 0 132 ± 1
Succinic acid (100 mM) 124 ± 2 135 ± 2
Britton-Robinson bufferc 136 ± 1 141 ± 1
a

Citrate-phosphate buffer according to reference 35.

b

Morpholinepropanesulfonic acid.

c

Described in reference 28.

d

Relative activities of LbBgl and BaBgl in organic and inorganic buffers at pH 7 were determined with pNP-β-d-glucopyranoside at 37°C. All values represent the means from triplicate determinations ± standard deviations.