TABLE 5.
Hydrolysis of DON-3G in aqueous cereal sample extracts
| Time (min) | Hydrolysis (mM) in extract ofa: |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Malt |
Wheat |
Rice |
Beerb |
Barleyc |
||||||
| DON-3G | DON | DON-3G | DON | DON-3G | DON | DON-3G | DON | DON-3G | DON | |
| 0 | 23 | ND | 22 | ND | 22 | ND | 23 | ND | 15 | 39 |
| 5 | ND | 21 | ND | 21 | <2.2 | 18 | 7.8 | 13 | ND | 50 |
| 10 | ND | 21 | ND | 20 | ND | 19 | <2.2 | 19 | ND | 50 |
| 15 | ND | 23 | ND | 21 | ND | 20 | <2.2 | 19 | ND | 49 |
a
Hydrolysis of DON-3G by BaBgl (0.8 mg ml−1) to DON in aqueous (100 mM Tris-Cl, pH 7) cereal sample extracts (1:5, wt/vol) spiked with 10 mg liter−1 (22 μM) DON-3G. All values represent the means from triplicate determination, and concentrations are displayed in millimolars. ND, not detectable (signal-to-noise ratio below 3:1).
b
Degassed and adjusted to pH 7 with 0.1 M KOH.
c
Naturally contaminated sample. Concentrations refer to dry sample (concentration in assay, 1:5).