TABLE 1.
PCR primers used for 16S rRNA gene and bgl sequence analyses
| Primer | Sequence (5′-3′) | Approximate length of product (bp) | Main applicationa |
|---|---|---|---|
| A 8-27fb | AGAGTTTGATCCTGGCTCAG | 1,476 | A |
| B 1523-1504rb | AAGGAGGTGATCCAGCCGCA | ||
| GRTF2 | CACCACCAATTTGTGGC | 47 | B |
| GRTR3 | GCTATCATACAGCCGATTT | ||
| GF4 | GCNYTNTTYGCNGARATGGG | 1,200 | C |
| GR4 | YTCNCCRTTNGTNGCDATNCA | ||
| GF5 | TGATCTGTTTGATGAGCTG | 801 | C |
| GR5 | AATGGGGTAGTCGTCCTG | ||
| GF6 | GCGCTGTTTGCTGAGATG | 799 | C |
| GR6 | ATGGGGTAGTCGTCCTGAA | ||
| GF7 | TTTGAAGAAGGAGAAAACAA | 427 | C |
| GR7 | ATCAATTTGCCAGCCCCA |
a
A, 16S rRNA gene PCR amplification; B, RNA-encoded β-d-glucosidase RT-PCR amplification; C, bgl PCR amplification.
b
Cited from Zhou et al. (14).