TABLE 2.
Details of engineered and natural haloalkaline environments that were compared in this study
| Environment type | Accession no. (database) | Sequencing method | Primer pair | pH | Salinity (ppt) | Source or reference |
|---|---|---|---|---|---|---|
| Engineered haloalkaline environments | ||||||
| Bauxite residue | SRP049788 (Sequence Read Archive) | 454 pyrosequencing | 27F/519R | 4–11 | 0.17–3.3b | This study |
| Oil sands tailings | SRP055527 (Sequence Read Archive) | 454 pyrosequencing | 27F/519R | 8.3 | 0.66b | This study |
| Steel slag | AY396008–AY396023 (GenBank)a | Sanger | 28F/1492R | 12 | 2.4–5.1 | 56 |
| Uranium mill tailings | HE650716–HE650774 (EMBL) | Ion Torrent pyrosequencing | 786F/926R | 10 | —c | 57 |
| Chromite ore processing residue | FR687640–FR687744 (EMBL) | Sanger | 8F/907R | 9–14 | 100 | 58 |
| Natural haloalkaline environments | ||||||
| Kenyan soda lakes | AJ517850–AJ517908 (EMBL) | Sanger | 27F/519R | 8.5–11.5 | 50–350 | 54 |
| Tirez Lagoon, Spain | EU734574, EU725589–EU725602, EU722643–EU722714, FJ172052–FJ172100, FJ236710–FJ236714 (GenBank) | Sanger | 27F/1492R | 7.3–9.08 | ≥69 | 55 |
a
Note that only sites 1, 2, and 4 returned DNA sequences.
b
Converted from dS m−1, assuming 1 dS m−1 = 0.550 ppt salinity.
c
—, data not available.