TABLE 5.
Phylogenetic associations of bacterial OTUs within bauxite residue samples and their matches to cultured representative sequences in the NCBI GenBank RefSeq 16S rRNA databasea
| Phylum and class | Total no. of OTUs | % of total OTUs | Phylum/class OTUs matched at >97% to existing cultured representatives (% of OTUs within sample) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| All samples | BR1 | BR2 | BR3 | BR4 | BR5 | BR6 | BR7 | BR8 | |||
| Proteobacteria | 1,820 | 50.40 | 11.76 | 50.00 | 58.82 | 47.06 | 63.64 | 11.49 | 5.68 | 14.27 | 9.60 |
| Alphaproteobacteria | 941 | 26.06 | 9.88 | 41.67 | 55.26 | 36.73 | 50.00 | 7.05 | 7.14 | 13.42 | 14.13 |
| Betaproteobacteria | 413 | 11.44 | 16.22 | 51.67 | 58.70 | 66.67 | 66.67 | 27.45 | 2.50 | 18.70 | 5.04 |
| Gammaproteobacteria | 290 | 8.03 | 16.90 | 75.00 | 84.00 | 53.33 | 100.00 | 36.00 | 4.17 | 18.13 | 4.84 |
| Deltaproteobacteria | 175 | 4.85 | 2.29 | 15.38 | 0.00 | 13.33 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Epsilonproteobacteria | 1 | 0.03 | 100.00 | 100.00 | 100.00 | —b | — | — | — | — | — |
| Actinobacteria | 549 | 15.20 | 13.84 | 57.69 | 72.73 | 52.38 | 64.71 | 8.11 | 11.31 | 14.35 | 7.69 |
| Bacteroidetes | 285 | 7.89 | 7.37 | 46.67 | 42.86 | 37.04 | 0.00 | 2.86 | 9.52 | 4.91 | 5.56 |
| Cytophagia | 138 | 3.82 | 2.17 | — | 0.00 | 0.00 | — | 0.00 | 7.69 | 1.23 | 5.00 |
| Sphingobacteria | 99 | 2.74 | 7.07 | 0.00 | 0.00 | 30.00 | — | 14.29 | 14.29 | 3.51 | 3.70 |
| Flavobacteria | 38 | 1.05 | 26.32 | 75.00 | 85.71 | 58.33 | 0.00 | 0.00 | — | 23.81 | 20.00 |
| Bacteroidia | 10 | 0.28 | 10.00 | 33.33 | 0.00 | 0.00 | 0.00 | — | 0.00 | 0.00 | — |
| Firmicutes | 199 | 5.51 | 29.65 | 73.53 | 78.57 | 34.78 | 75.00 | 11.11 | 23.81 | 19.15 | 7.14 |
| Bacilli | 117 | 3.24 | 41.03 | 80.00 | 81.82 | 66.67 | 80.00 | 14.29 | 28.30 | 36.36 | 25.00 |
| Clostridia | 77 | 2.13 | 14.29 | 55.56 | 66.67 | 14.81 | 50.00 | 0.00 | 0.00 | 4.35 | 0.00 |
| Erysipelotrichi | 5 | 0.14 | 0.00 | — | — | 0.00 | — | 0.00 | — | 0.00 | 0.00 |
| Chloroflexi | 172 | 4.76 | 1.16 | — | — | 0.00 | 0.00 | 0.00 | 2.38 | 0.00 | 0.00 |
| Planctomycetes | 155 | 4.29 | 1.29 | 0.00 | — | 0.00 | — | 0.00 | 0.00 | 2.13 | 0.00 |
| Acidobacteria | 132 | 3.66 | 0.00 | — | — | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Gemmatimonadetes | 78 | 2.16 | 0.00 | 0.00 | — | 0.00 | — | 0.00 | 0.00 | 0.00 | 0.00 |
| Verrucomicrobia | 47 | 1.30 | 0.00 | 0.00 | — | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Totals | 3,437 | 95.18 | 10.50 | 52.27 | 60.32 | 38.78 | 60.19 | 8.07 | 7.85 | 10.78 | 7.52 |
a
Phyla representing <1% of total OTUs were excluded from this table.
b
—, no OTUs from this phylum were present in this sample.