TABLE 2.
LD50s of the virulence attenuation mutants in this study
| Disrupted genea | Description of gene | LD50 (CFU) | Virulence attenuation (fold)b |
|---|---|---|---|
| AS87_08795 | Gliding-motility protein GldM | 1.97 × 107 | 184 |
| AS87_01220 | Amidophosphoribosyltransferase | 2.88 × 107 | 269 |
| AS87_01210 | Phosphoribosylformylglycinamidine (FGAM) synthase | 5.75 × 107 | 537 |
| AS87_00250 | PorT protein | 1.24 × 108 | 1,160 |
| AS87_01015 | Glycosyltransferase family 2 | 1.27 × 108 | 1,190 |
| AS87_00910 | Phosphoribosylaminoimidazole (AIR) synthetase | 1.48 × 108 | 1,380 |
| AS87_04980 | Antitermination protein NusB | 2.43 × 108 | 2,270 |
| AS87_00505 | Gliding-motility protein GldG | 3.16 × 108 | 2,950 |
| AS87_05585 | Beta-lactamase | 3.16 × 108 | 2,950 |
| AS87_07625 | Fructose-bisphosphate aldolase | 4.16 × 108 | 3,890 |
| AS87_04765 | Beta-carotene 15,15′-monooxygenase | 4.27 × 108 | 3,990 |
| AS87_08785 | Gliding-motility protein Gldk | 7.5 × 108 | 7,000 |
| AS87_03285 | Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase) | 1 × 109 | 9,300 |
| AS87_06385 | Transcriptional regulator, LuxR family | 3.83 × 109 | 35,800 |
| AS87_04750 | Inosine-5-monophosphate dehydrogenase | 8.13 × 109 | 76,000 |
| AS87_09295 | Pyruvate dehydrogenase | 1 × 1010 | 93,400 |
| AS87_10020 | Hypothetical protein | 1.10 × 1010 | 103,000 |
| AS87_09440 | ATPase | 1.76 × 1010 | 165,000 |
| AS87_04050 | Vi polysaccharide biosynthesis protein VipB/TviC | 1.91 × 1010 | 179,000 |
| AS87_01735 | PncA | 5.23 × 1010 | 488,000 |
| AS87_09530 | 8-Amino-7-oxononanoate synthase | 8.22 × 1010 | 768,000 |
| AS87_05545 | Biotin synthase | 2.18 × 1011 | 2,040,000 |
a
Based on the R. anatipestifer Yb2 genome (accession no. CP007204).
b
The measured LD50 of wild-type strain Yb2 was 1.07 × 105 CFU. Fold virulence attenuation was calculated by dividing the LD50 of the mutant strain by the LD50 of wild-type strain Yb2.