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Phylogenetic tree of classic HAstV and VA-AstV strains obtained using an approximately 360-nt fragment at the 5′ end of ORF2. Strains detected from influent samples (marked ●) and strains detected from effluent samples (marked ○), along with corresponding historical AstV strains, including MLB-AstVs and other animal AstVs strains, are included. Bootstrap values greater than 700 among 1,000 replicates are shown on each branch.
