TABLE 2.

BLASTX analysis of nonribosomal peptide synthetase putative sequences obtained through PCR-based screening of the Sphagnum moss metagenomic fosmid library^a

Clone ID (query)	Primer pair (screening)	Close hit (accession no.)	Description [source]	E value	Score	Bit score	Alignment length (aa)	% identity	% positivity	% gaps
3-F3	NRPS1	WP_008434512	Peptide synthase [Pseudomonas sp. strain Ag1]	0	1,691	656	345	99.4	99.4	0
		WP_005745216	Pyoverdine side-chain peptide synthetase III, partial [Pseudomonas amygdali]	4.76E−131	1,019	397	342	60.6	73.9	0.87
7-B9	NRPS2	WP_031373175	Hypothetical protein, partial [Lysobacter antibioticus]	6.26E−116	969	378	320	62.9	73.8	1.25
		WP_032634710	Nonribosomal peptide synthetase module(s), partial [Pseudomonas syringae]	8.94E−105	845	330	322	57.2	68.0	3.08
6-H4	NRPS2	WP_031447884	Peptide synthetase [Caulobacteraceae bacterium PMMR1]	3.68E−114	948	370	301	64.1	76.1	0.66
2-F4	NRPS1	WP_032630858	Hypothetical protein, partial [Pseudomonas syringae]	4.78E−88	723	283	254	64.2	76.0	1.18
		WP_004417722	Syringomycin synthetase E, partial [Pseudomonas syringae]	2.55E−73	651	255	255	57.3	69.4	1.18
7-F1	NRPS2	WP_031447884	Peptide synthetase [Caulobacteraceae bacterium PMMR1]	1.47E−54	498	196	147	73.5	81.0	0
4-B4	NRPS2	WP_013428427	Peptide synthetase [Burkholderia rhizoxinica]	5.34E−48	451	178	143	70.1	78.5	0.69
		WP_015833634	Syringopeptin synthetase b [Photorhabdus asymbiotica]	2.32E−41	400	158	143	64.6	74.3	0.69
7-D4	NRPS2	AHX13665	Thioester reductase [Dyella jiangningensis]	1.10E−43	418	166	126	62.7	77.8	0
		WP_042439855	Gramicidin synthetase LgrC, partial [Streptacidiphilus albus]	2.08E−29	304	122	122	50	66.4	0.82
2-D4	NRPS1	WP_030110998	Hypothetical protein, partial [Kutzneria albida]	2.30E−41	415	165	209	47.9	58.1	3.72
8-C8	NRPS2	WP_011473791	Amino acid adenylation [Rhodopseudomonas palustris]	6.93E−34	346	138	156	50.3	61.6	5.03
6-B1	NRPS2	AHX13666	Amino acid adenylation protein [Dyella jiangningensis]	5.51E−31	323	129	103	72.8	80.6	0
3-H3	NRPS1	WP_010564295	Peptide synthetase [Pseudomonas extremaustralis]	1.56E−18	218	89	45	91.1	95.6	0
3-G9	NRPS1	CDG17982	Nonribosomal peptide synthetase [Xenorhabdus doucetiae]	9.97E−10	151	63	39	74.4	76.9	0
2-C8	NRPS1	WP_022609339	Nonribosomal peptide synthetase module, partial [Rubidibacter lacunae]	2.29E−08	138	58	29	86.2	89.7	0
7-C3	NRPS2	WP_032631609	Hypothetical protein, partial [Pseudomonas syringae]	0.05	88	39	20	90.0	90.0	0

^aFor fosmid clones 3-F3, 3-H3, 2-D4, 2-F4, 7-B9, and 6-H4, longer sequences (759 to 1,155 bp, 252 to 384 aa) were retrieved by primer walking. BLASTX searches were performed against the nonredundant protein database (http://www.ncbi.nlm.nih.gov/protein; 7 April 2015). ID, identifier; NRPS, nonribosomal peptide synthetase.