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. 2015 Jul 8;35(27):9966–9976. doi: 10.1523/JNEUROSCI.0337-15.2015

Figure 6.

Figure 6.

TLR2-activated macrophages create a microenvironment capable of stabilizing dystrophic axons after SCI. Confocal montages of longitudinal spinal cord sections 4 dpi. A–F, Vimentin expression is increased 2 d after intraspinal injection (1 μl) of TLR2 agonist Pam2CSK4 (D–F) compared with vehicle injection (A–C). Edge of the lesion center in A and D labeled with asterisks. G, The density of vimentin labeling in proximity to labeled axons is significantly increased in spinal cords injected with the TLR2 agonist (**p < 0.01, n = 3/group). H–M, High-powered confocal projections show more axons closely associated with vimentin-positive cells (arrows) in spinal cords injected with TLR2 agonist. Arrowheads depict axons that are not associated with vimentin. N, Significantly more axons were apposed to vimentin-positive cells following injection of the TLR2 agonist (***p < 0.001, n = 3/group). O–T, Confocal images reveal vimentin colocalization with NG2-positive (O–Q) but not ED-1-positive (R–T) cells (z-plane to the right and bottom of each image). Data are representative of two independent experiments. Scale bars: A–F, 100 μm; H–M, 50 μm; O–T, 10 μm.