Figure 4.

UNC-7 diminishes the magnitude of spicule insertion attempts at the vulva and restricts intromission attempts at nonvulva regions. Mating observations between wild-type and unc-7(rg396) males. A, Mating potency for wild-type and unc-7(rg396) with moving hermaphrodites. Numbers on top and bottom of the bars indicate percentages and number of males tested. The p value was calculated using Fisher's exact t test. B, The percentage of time that males spent in contact with the vulva out of 120 s of total contact. Each symbol represents an individual's male performance. Open symbols indicate males that fully protracted their spicules out of the tail. C, D, VIP during a 60 s observation window of mating with nonmoving hermaphrodites. C, Comparison of wild-type and unc-7(rg396) mutants. D, Comparison of control and UNC-7(L) (A59T) overexpression from the dop-2 promoter. E, A representative trace of pixel fluxes during spicule insertion attempts of a wild-type (red trace) and mutant male (blue trace) for 6 s. F, Average SD of pixel fluxes per second for 5 males per group. For each male, their prodding duration was broken down to five periods. Each point represents the SD of pixel fluxes in a 1 s period (B–F). Red line indicates the average value for each group. The p value was calculated using the Mann–Whitney nonparametric test. The exact p values are indicated above the black connector lines.