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. 2015 Jul 8;3:44. doi: 10.3389/fcell.2015.00044

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Copyright © 2015 Reiten, Bakkebø, Brun-Hansen, Lewandowska-Sabat, Olsaker, Tranulis, Espenes and Boysen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phagocytosis assays. Cytospots confirmed the cellular uptake of (A) latex beads (green fluorescence), (B) E. coli (red fluorescence), and (C) Zymosan (red fluorescence) in activated monocytes. Additional staining for nuclei (blue) and CD68 (A red, B and C green). (D) Gating of live cells based on FS and SS characteristics in flow cytometry. (E) Particle uptake in PRNP^Ter/Ter and PRNP^+/+ cells based on the results from two representative animals. Medium only was used as control. Gates indicate particle-containing cells, and in the case of latex beads, also gates for cells that had engulfed 2 particles or more (F–H) Compiled results of all animals showing percentage of monocytes containing (F) latex beads, (G) Zymosan and (H) E. coli, as measured by flow cytometry. For each assay, n = 8, except PRNP^Ter/Ter E. coli where n = 7.