Figure 1.

IBC cells survive in ECM-detached conditions. (a) Cells were plated on either normal or poly-HEMA coated plates, and caspase activation was measured at 48 h using the Caspase-Glo assay. (b) Cells were plated in attached or detached conditions, and cell viability was measured at 48 h post-plating via Cell Titer Glo Assay. (c) Cells were plated on either normal or poly-HEMA coated plates, and cell viability was measured at the indicated times via alamarBlue assay. (d) MCF-10A and SUM149 cells were plated at 100 000 cells/well, and KPL-4 cells were plated at 60 000 cells/well in soft agar. Colonies were stained at either 14 days (KPL-4) or 30 days (SUM149, MCF-10A) using 0.01% INT-violet and quantified with ImageJ. Error bars represent S.E.M. *P-value<0.05 compared with MCF-10A