Figure 4.

MEK inhibition does not affect Bcl-2 family member protein levels in IBC cells. (a) Cells were plated on poly-HEMA-coated plates with DMSO or increasing amounts of U0126 for 48 h, and protein content was analyzed via immunoblotting. (b) Cells were plated in ECM-detached conditions with DMSO or U0126 (10 μM) for 48 h. Cell lysates were prepared for immunoblotting. (c) Cells were plated on poly-HEMA-coated plates with DMSO or PD0325901 (1 μM) for 48 h, and lysates were prepared for immunoblotting. (d) Seven IBC patient tissue samples were obtained, and IHC was utilized to visualize Bim protein levels in the tissue. Images were blind scored for staining intensity. (e) Cells were plated in detachment and treated with DMSO or increasing amounts of U0126 for 48 h. Lysates were prepared and normalized, and protein levels were analyzed via immunoblotting. (f) KPL-4 and SUM149 cells were plated in detachment with the indicated doses of U0126 or PD0325901 for 48 h. Protein levels were analyzed via western blotting