Figure 2.

Lack of p73 affects TGF-β signaling in vivo. Quantification of VEGF-A expression and analysis of the TGF-β/ALK1/ID1 signaling pathway in P5 retinas from WT and p73KO mice. qRT-PCR analysis demonstrated a significant decrease in VEGF-A expression (a), TGF-β 1 expression (b), TGF-βR1 expression (c), ALK1 expression (d) and ID1 expression levels (e) in the absence of p73. Analysis was performed with data from three independent experiments. Mean±S.D. are represented; equal-variance Student's t-test was performed to evaluate statistical differences. *P<0.05, **P<0.01, ***P<0.001