Figure 5.

p73 function, and DNp73 in particular, is required for tube morphogenesis in HUVEC. Tube formation assays on Matrigel in HUVEC upon (a) p73 functional inhibition – DDp73, (b) knockdown of total p73 – p73i.4 or (c) specific knockdown of TA or DNp73 isoforms – Tap73i and DNp73i, respectively. Tube formation was monitored microscopically and images were analyzed using WimTube software (Wimasis GmbH). Covered area, total tubes, total tube length, total branching points and total number of loops are shown as representative parameters. Experimental data were normalized to the control (a) or to the scrambled (Scr.; b,c). Data represent mean values±S.D.; n≥3, experiments were repeated at least three times; equal-variance Student's t-test was performed to evaluate statistical differences: *P<0.05, **P<0.01, ***P<0.001