Figure 6.

p73, and DNp73 in particular, acts as a positive regulator of HUVEC migration. Wound healing assays upon (a) p73 functional inhibition (DDp73, upper panel) and total p73 knockdown (p73i.4, lower panel) or (c) specific knockdown of TA or DNp73 (TAp73i and DNp73i, respectively). Endothelial cell migration into the ‘wound' denuded area was monitored microscopically and the percentage of wound closure after 10 h was calculated relative to the control. (b) Expression kinetics of p73 isoforms during wound-healing assays were analyzed by qRT-PCR. (d) HUVEC were cotransfected with p73i4 siRNA (silencing of total p73) or scrambled oligos (Scr.) together with a DNp73 expressing plasmid, and a wound healing assay was performed. Data represent mean values±S.D.; n=3; experiments were repeated at least three times; equal-variance Student's t-test was performed to evaluate statistical differences. *P<0.05, **P<0.01, ***P<0.001