Figure 3.

Conditioned medium containing secreted LAMC2 enhances cell migration and invasion, which can be blocked by LAMC2-blocking antibody. (a) Immunoblot analysis using the concentrated conditioned medium from LAMC2-transfected and -knockdown cells identified secreted unprocessed (140 kDa) and processed (100 kDa) LAMC2. Ponceau-S staining of the membrane is shown as protein loading controls of the conditioned medium. (b) Migration and invasion assay upon addition of conditioned medium from shLAMC2- and shMock-transfected cells to PC9-shLAMC2. Student's t-test was performed. Data are presented as mean±S.E.M. of triplicate experiments. (c) Migration and invasion assay upon addition of mixture of LAMC2-blocking antibody and LAMC2-containing conditioned medium from A549R0-LAMC2 cells, to A549R0-Mock cells. mAb was the blocking monoclonal antibody and immunoglobulin G (IgG) was used as the control. Student's t-test was performed. Data are presented as mean±S.E.M. of triplicate experiments